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on 02 June 2009. After hormone ejections, sperm th collected from each male at 28 hours. To avoid any sperm contamination by urine, the urinary bladder emptied by gently squeezing the fish belly. T...
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solutions buffered with Tris-HCI are used as extenders according to Billard et al. (2004) and Kopeika et al. (2007). The different extenders are shown at Table 1. The highest post-thaw sperm motili...
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